Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica
نویسندگان
چکیده
منابع مشابه
Agrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
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The study was conducted to standardize a protocol for Agrobacterium-mediated genetic transformation of buffel grass (Cenchrus ciliaris L.). Embryogenic calli, produced from one-year-old mature seeds of buffel grass, were used as target cells for Agrobacterium-mediated transformation. A. tumefaciens strain LBA4404, harbouring pCAMBIA-1301 or pCAMBIA-2301, was used for co-cultivation with embryog...
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15 صفحه اولagrobacterium-mediated transformation of pomegranate (punica granatum l.) ‘yousef khani’ using the gus reporter gene
in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
متن کاملWild-type Agrobacterium rhizogenes-mediated gene transfer in plants: Agrobacterium virulence and selection of transformants
Agrobacterium rhizogenes ATCC 15834 wild type strain was transformed with the binary vector pBI121 using the heat shock method. The transformed Agrobacterium was then tested for virulence through tobacco leaf explant transformation. Compared to the non-transformed Agrobacterium, the transformed Agrobacterium showed reduced virulence, producing signifi cantly lower number of hairy roots in tobac...
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ژورنال
عنوان ژورنال: Journal of Agricultural Biotechnology and Sustainable Development
سال: 2014
ISSN: 2141-2340
DOI: 10.5897/jabsd2013.0218